5HGT
Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92T at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2013-11-11 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.024, 60.424, 37.891 |
Unit cell angles | 90.00, 93.76, 90.00 |
Refinement procedure
Resolution | 37.810 - 1.750 |
R-factor | 0.1756 |
Rwork | 0.173 |
R-free | 0.21240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.017 |
RMSD bond angle | 1.815 |
Data reduction software | APEX 2 |
Data scaling software | APEX 2 |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.022 | 0.184 |
Total number of observations | 171579 | |
Number of reflections | 14161 | |
<I/σ(I)> | 31.33 | 5.08 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.06 | 5.81 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |