5HGR
Structure of Anabaena (Nostoc) sp. PCC 7120 Orange Carotenoid Protein binding canthaxanthin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 52.989, 53.371, 65.043 |
Unit cell angles | 93.23, 112.93, 93.09 |
Refinement procedure
Resolution | 38.116 - 1.681 |
R-factor | 0.1709 |
Rwork | 0.170 |
R-free | 0.20170 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.835 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.116 | 1.770 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.105 | 0.565 |
Number of reflections | 70766 | |
<I/σ(I)> | 8.1 | 2.5 |
Completeness [%] | 95.1 | 86.1 |
Redundancy | 3.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM sodium formate, pH 6, 10% PEG 3350 |