5HGN
Hexameric HIV-1 CA, apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 6 |
Unit cell lengths | 90.730, 90.730, 56.751 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.690 - 1.900 |
R-factor | 0.2013 |
Rwork | 0.200 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h4e |
RMSD bond length | 0.007 |
RMSD bond angle | 0.998 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.4.2) |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 26.690 | 26.690 | 1.940 |
High resolution limit [Å] | 1.900 | 8.910 | 1.900 |
Rmerge | 0.121 | 0.074 | 0.748 |
Rpim | 0.059 | 0.035 | 0.379 |
Total number of observations | 106747 | 1165 | 6748 |
Number of reflections | 21070 | ||
<I/σ(I)> | 8.3 | 17.1 | 2.1 |
Completeness [%] | 99.8 | 96.9 | 100 |
Redundancy | 5.1 | 5.4 | 4.8 |
CC(1/2) | 0.992 | 0.994 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | PEG550MME (13-14%), KSCN (0.15M), TRIS (0.1M, pH 8.5) |