5HGH

HLA*A2402 complexed with HIV nef138 10mer epitope

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-03-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	C 1 2 1
Unit cell lengths	161.735, 65.062, 50.415
Unit cell angles	90.00, 90.23, 90.00

Refinement procedure

Resolution	42.706 - 2.392
R-factor	0.1901
Rwork	0.188
R-free	0.22230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bck
RMSD bond length	0.003
RMSD bond angle	0.689
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.392	2.392
Rmerge		0.469
Number of reflections	20191
<I/σ(I)>	19.968	2.642
Completeness [%]	97.2	92.6
Redundancy	4.1	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M MES monohydrate pH 6.5, 12% w/v Polyethylene glycol 20000