5HGE

Filamentous Assembly of Green Fluorescent Protein Supported by a C-terminal fusion of 18-residues, viewed in space group P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	51.160, 62.910, 69.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.570 - 1.863
R-factor	0.1731
Rwork	0.169
R-free	0.21140
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4piq
RMSD bond length	0.010
RMSD bond angle	1.130
Data scaling software	Aimless (0.5.17)
Phasing software	BUSTER
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.570	1.869
High resolution limit [Å]	1.863	1.863
Rmerge	0.106	0.557
Rpim	0.060	0.317
Total number of observations	69078	10135
Number of reflections	18522
<I/σ(I)>	9.4	2
Completeness [%]	96.0	96.6
Redundancy	3.7	3.8
CC(1/2)	0.997	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	30% MPD, 20% ethanol, 20 mM HEPES, pH 7.5, 10 mM NaCl