5HGA
HLA*A2402 complex with HIV nef138 Y2F-8mer mutant epitope
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-03-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.838, 86.913, 151.441 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.860 - 2.199 |
R-factor | 0.2075 |
Rwork | 0.205 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bck |
RMSD bond length | 0.006 |
RMSD bond angle | 1.055 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.199 | 2.199 |
Rmerge | 0.659 | |
Number of reflections | 324304 | |
<I/σ(I)> | 24.39 | 2.588 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 7.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 291 | 0.1M MES monohydrate pH 6.5, 12% w/v Polyethylene glycol 20000 |