5HFL
Gp41-targeting HIV-1 fusion inhibitors with helical Ile-Asp-Leu tail
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.449, 114.471, 42.936 |
| Unit cell angles | 90.00, 91.80, 90.00 |
Refinement procedure
| Resolution | 42.915 - 2.294 |
| R-factor | 0.2194 |
| Rwork | 0.214 |
| R-free | 0.26290 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.447 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.915 |
| High resolution limit [Å] | 2.294 |
| Number of reflections | 17227 |
| <I/σ(I)> | 10.5 |
| Completeness [%] | 99.6 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.2 | 289 | 0.2 M NaCl, 0.1 M Na2HPO4, citric acid, 15-20%(w/v) PEG 3000 |
