5HFC
The third PDZ domain from the synaptic protein PSD-95 (H372A mutant) in complex with a mutant C-terminal peptide derived from CRIPT (T-2F)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-09-11 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 89.445, 89.445, 89.445 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.001 - 1.851 |
R-factor | 0.1865 |
Rwork | 0.184 |
R-free | 0.20730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1be9 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.462 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10pre_2104) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.001 | 1.936 |
High resolution limit [Å] | 1.851 | 1.851 |
Rmerge | 0.040 | 0.448 |
Number of reflections | 10760 | |
<I/σ(I)> | 35.543 | 1 |
Completeness [%] | 98.2 | 88 |
Redundancy | 6.3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.05 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (7 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer. |