5HF4
The third PDZ domain from the synaptic protein PSD-95 (H372A mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792062 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 89.254, 89.254, 89.254 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.916 - 1.750 |
| R-factor | 0.1861 |
| Rwork | 0.182 |
| R-free | 0.22070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bfe |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.928 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2026) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.916 | 1.820 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.045 | |
| Number of reflections | 12757 | |
| <I/σ(I)> | 48.917 | 2.286 |
| Completeness [%] | 99.6 | |
| Redundancy | 11.4 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | Reservoir solution contained 0.95 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer. |
