5HEW
Pentameric ligand-gated ion channel ELIC mutant T28D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.603 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.450, 266.960, 110.200 |
| Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
| Resolution | 29.929 - 4.500 |
| R-factor | 0.2387 |
| Rwork | 0.237 |
| R-free | 0.27070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yn6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.584 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.600 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.093 | 1.892 |
| Number of reflections | 33795 | |
| <I/σ(I)> | 13.1 | 1.9 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 11.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG 4000 ammonium sulfate |
