5HEH
Pentameric ligand-gated ion channel GLIC mutant P246A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9197 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.690, 133.940, 159.830 |
| Unit cell angles | 90.00, 101.10, 90.00 |
Refinement procedure
| Resolution | 29.913 - 3.300 |
| R-factor | 0.2457 |
| Rwork | 0.244 |
| R-free | 0.28030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ehz |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.654 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.101 | 0.773 |
| Number of reflections | 55197 | |
| <I/σ(I)> | 13.1 | 2.4 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | PEG 4000, Ammonium Sulfate |
