5HEG
Pentameric ligand-gated ion channel GLIC mutant P246G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.380, 134.210, 160.790 |
| Unit cell angles | 90.00, 101.97, 90.00 |
Refinement procedure
| Resolution | 29.924 - 3.214 |
| R-factor | 0.2476 |
| Rwork | 0.247 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ehz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.777 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.122 | 0.956 |
| Number of reflections | 60901 | |
| <I/σ(I)> | 9.3 | 9.3 |
| Completeness [%] | 98.3 | 82.8 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | Ammonium sulfate, PEG 4000 |
