5HDW
ApaG Domain of FBxo3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2014-08-03 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 30.477, 96.813, 40.517 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.380 - 2.000 |
| R-factor | 0.2036 |
| Rwork | 0.200 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xvs |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.329 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.380 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 | 0.417 |
| Number of reflections | 8588 | |
| <I/σ(I)> | 10.3 | 2.7 |
| Completeness [%] | 99.3 | 97.8 |
| Redundancy | 5.41 | 5.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 100mM Tris-HCl pH 8.5 20% w/v PEG 8000 200mM MgCl2 * 6H2O |
