5HDT
Human cohesin regulator Pds5B bound to a Wapl peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 120.760, 162.368, 173.061 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.600 - 2.711 |
R-factor | 0.217 |
Rwork | 0.216 |
R-free | 0.25330 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.621 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.600 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.095 | 1.000 |
Number of reflections | 92470 | |
<I/σ(I)> | 18.4 | 1.2 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 8.1 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M Sodium Citrate and 20% PEG3350 (vol/vol) without buffer or with buffer 0.1 M Bis-Tris propane pH6.5, pH 7.5 or pH 8.5 |