5HDQ
MntC co-structure with mAB 305-78-7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 179.712, 41.733, 108.404 |
Unit cell angles | 90.00, 97.09, 90.00 |
Refinement procedure
Resolution | 36.600 - 1.830 |
R-factor | 0.2086 |
Rwork | 0.207 |
R-free | 0.23640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qgc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.230 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.9.7) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.600 |
High resolution limit [Å] | 1.830 |
Number of reflections | 43860 |
<I/σ(I)> | 1 |
Completeness [%] | 61.6 |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Protein complex (10 mM HEPES pH 7.5 and 150 mM NaCl) was concentrated to 5.16 mg/mL and crystals grown by the hanging drop method using 1 M LiCl, 0.1 M MES, pH 6.0 and 20% PEG 6K as the precipitating reagents. |