5HDN
Crystal structure of heat shock factor1-DBD complex with ds-DNA and TtT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97845 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.415, 127.367, 55.268 |
| Unit cell angles | 90.00, 100.55, 90.00 |
Refinement procedure
| Resolution | 41.334 - 1.680 |
| R-factor | 0.1755 |
| Rwork | 0.174 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hdg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.943 |
| Data reduction software | XSCALE |
| Data scaling software | XDS |
| Phasing software | XDS |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.334 | 1.720 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.047 | 0.477 |
| Number of reflections | 118571 | |
| <I/σ(I)> | 15.68 | 3 |
| Completeness [%] | 98.4 | 88.6 |
| Redundancy | 5.7 | 2.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 293 | 6%v/v Ethylene glycol, 0.1M citric acid PH3.8, 10%w/v PEG6000. |
