5HDL
Crystal structure of shaft pilin spaA from Lactobacillus rhamnosus GG - E269A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 228.632, 63.033, 104.904 |
| Unit cell angles | 90.00, 95.34, 90.00 |
Refinement procedure
| Resolution | 80.790 - 2.390 |
| R-factor | 0.22002 |
| Rwork | 0.219 |
| R-free | 0.23691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f44 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.185 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.790 | 2.670 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.066 | 0.605 |
| Number of reflections | 59176 | |
| <I/σ(I)> | 16 | 2.9 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M tri-Potassium citrate, 20% (w/v) PEG 3350 |
