5HDG
crystal structure of heat shock factor 1-DBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 48.610, 64.420, 68.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.210 - 1.700 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hts |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.753 |
| Data reduction software | XSCALE |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.210 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.120 | 0.527 |
| Number of reflections | 12343 | |
| <I/σ(I)> | 4.1 | 3 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.2M sodium formate, pH 7.2, 22%(w/v) PEG3350 |
