5HDE
Crystal Structure of PTPN12 Catalytic Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.010, 40.967, 75.686 |
| Unit cell angles | 90.00, 116.63, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.620 |
| R-factor | 0.16659 |
| Rwork | 0.164 |
| R-free | 0.21265 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qcj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.459 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.620 |
| Number of reflections | 47713 |
| <I/σ(I)> | 37.7 |
| Completeness [%] | 99.9 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.04M potassium phosphate, 16% w/v PEG 8000, 20% v/v glycerol |
