5HDA
Crystal Structure of the BS69 coiled coil-MYND domains bound to an EBNA2 PXLXP motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.456, 38.190, 82.690 |
| Unit cell angles | 90.00, 97.53, 90.00 |
Refinement procedure
| Resolution | 44.600 - 2.390 |
| R-factor | 0.2241 |
| Rwork | 0.222 |
| R-free | 0.26160 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.874 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.650 | 2.490 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.096 | 0.362 |
| Number of reflections | 14546 | |
| <I/σ(I)> | 12.4 | 2.3 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 21% PEGMME 350, 0.1M Tris-HCl pH 8.0, 6% Methanol, Hanging Drop Vapor Diffusion, Temperature 277K |
