5HCR

Crystal structure of antimicrobial peptide Oncocin 10wt bound to the Thermus thermophilus 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	209.833, 450.806, 622.717
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.620 - 2.800
R-factor	0.2177
Rwork	0.215
R-free	0.26810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.971
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	49.776	2.970
High resolution limit [Å]	2.800	8.290	2.800
Rmerge	0.221	0.034	1.645
Rmeas	0.246	0.038	1.836
Total number of observations	7075688
Number of reflections	1417926	55829	224957
<I/σ(I)>	9.54	34.78	1.01
Completeness [%]	99.0	98	97.8
Redundancy	4.99
CC(1/2)		0.999	0.260

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME