5HCP
Crystal structure of antimicrobial peptide Metalnikowin bound to the Thermus thermophilus 70S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97910 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.873, 450.493, 623.112 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.848 - 2.894 |
R-factor | 0.2148 |
Rwork | 0.212 |
R-free | 0.26780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.967 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 49.848 | 3.070 |
High resolution limit [Å] | 2.890 | 8.570 | 2.890 |
Rmerge | 0.216 | 0.032 | 1.241 |
Rmeas | 0.241 | 0.035 | 1.401 |
Total number of observations | 6250510 | ||
Number of reflections | 1277977 | 50682 | 197092 |
<I/σ(I)> | 9.07 | 38.06 | 1.24 |
Completeness [%] | 98.6 | 98 | 94.7 |
Redundancy | 4.891 | ||
CC(1/2) | 0.999 | 0.357 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 292 | 0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME |