5HCM

The GLIC pentameric Ligand-Gated Ion Channel 2-21' cross-linked mutant complexed to bromoform

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-01-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91840
Spacegroup name	C 1 2 1
Unit cell lengths	177.268, 130.934, 155.760
Unit cell angles	90.00, 100.31, 90.00

Refinement procedure

Resolution	31.750 - 3.150
R-factor	0.2308
Rwork	0.230
R-free	0.24010
RMSD bond length	0.008
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.000
High resolution limit [Å]	3.150
Number of reflections	60505
<I/σ(I)>	13
Completeness [%]	99.7
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291	PEG 4000 12-15%, 0.1 M Na Acetate pH4, 0.4M NaSCN, 15% glycerol