5HCJ
Cationic Ligand-Gated Ion Channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91840 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.944, 133.372, 159.337 |
| Unit cell angles | 90.00, 102.34, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.950 |
| R-factor | 0.2138 |
| Rwork | 0.213 |
| R-free | 0.21850 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.950 |
| Number of reflections | 77979 |
| <I/σ(I)> | 11.8 |
| Completeness [%] | 99.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | PEG4000 12-15%, NaSCN 0.4M, 15% glycerol, NaAcetate pH 4 0.1M |
