5HCG
CRYSTAL STRUCTURE OF FREE-CODV.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.920, 70.920, 272.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.160 - 2.680 |
| R-factor | 0.2317 |
| Rwork | 0.229 |
| R-free | 0.29650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CODV part of 5FHX |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 2.830 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Number of reflections | 20335 | |
| <I/σ(I)> | 32.7 | 4.8 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 12.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | HEPES 0,1M, PEG 2000 MME 17.5% |
