5HC9
Thermotoga maritima CCA-adding enzyme complexed with tRNA_CCA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-12-11 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.180, 96.190, 108.580 |
Unit cell angles | 90.00, 110.30, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.2171 |
Rwork | 0.215 |
R-free | 0.26390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h38 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.638 |
Data reduction software | XSCALE |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 | |
High resolution limit [Å] | 2.900 | 12.960 | 2.900 |
Rmerge | 0.203 | 0.036 | 2.273 |
Rmeas | 0.217 | 0.039 | 2.588 |
Total number of observations | 322538 | ||
Number of reflections | 42228 | 480 | 3035 |
<I/σ(I)> | 10.26 | 46.25 | 1.1 |
Completeness [%] | 99.6 | 93.4 | 98.3 |
Redundancy | 7.6 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 8000, magnesium chloride, Tris-Cl |