5HBX
RNA primer-template complex with 2-methylimidazole-activated monomer analogue-2 binding sites
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-18 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.987 |
Spacegroup name | P 3 |
Unit cell lengths | 43.545, 43.545, 83.996 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.21478 |
Rwork | 0.213 |
R-free | 0.24899 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dhc |
RMSD bond length | 0.026 |
RMSD bond angle | 2.770 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.128 | 0.488 |
Number of reflections | 19566 | |
<I/σ(I)> | 14.6 | 2.64 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.4 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | Ammonium acetate, Magnesium chloride hexahydrate,0.05 M TRIS hydrochloride pH 7.5,10% v/v (+/-)-2-Methyl-2,4-pentanediol |