5HBD

Filamentous Assembly of Green Fluorescent Protein Supported by a C-terminal fusion of 18-residues, viewed in space group C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	112.700, 51.340, 55.060
Unit cell angles	90.00, 99.64, 90.00

Refinement procedure

Resolution	55.620 - 1.650
R-factor	0.17628
Rwork	0.173
R-free	0.20475
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.696
Data scaling software	XSCALE (Oct 15, 2015)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.620		1.750
High resolution limit [Å]	1.650	4.920	1.650
Rmerge	0.094	0.036	0.534
Rmeas	0.103	0.040	0.645
Total number of observations	205218
Number of reflections	34226	1463	3851
<I/σ(I)>	13.65	35.31	2.29
Completeness [%]	91.2	98.4	63.8
Redundancy	6		2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M sodium citrate, 10% MPD, 0.1M HEPES, pH 7.5