5HB8
Crystal structure of Chaetomium thermophilum Nup53 RRM (space group P3121)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 94.660, 94.660, 115.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.330 - 1.700 |
R-factor | 0.1643 |
Rwork | 0.163 |
R-free | 0.21010 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.999 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.700 |
Number of reflections | 60735 |
<I/σ(I)> | 37.3 |
Completeness [%] | 91.1 |
Redundancy | 35.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 4 % Tacsimate (pH 4.1), 15 % (w/v) PEG 3350 |