5HB5

Crystal structure of Chaetomium thermophilum Nup145N APD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	46.082, 34.856, 78.179
Unit cell angles	90.00, 99.37, 90.00

Refinement procedure

Resolution	27.663 - 1.500
R-factor	0.1627
Rwork	0.161
R-free	0.18860
RMSD bond length	0.005
RMSD bond angle	0.808
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.500
Number of reflections	38743
<I/σ(I)>	12.4
Completeness [%]	97.2
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1 M citric acid (pH 3.0), 25 % (w/v) PEG 3350