5HB3
Crystal structure of Chaetomium thermophilum Nic96 SOL-Nup53 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 59.520, 86.950, 98.010 |
Unit cell angles | 100.69, 99.59, 95.66 |
Refinement procedure
Resolution | 47.285 - 2.650 |
R-factor | 0.2134 |
Rwork | 0.212 |
R-free | 0.24880 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.562 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.650 |
Number of reflections | 48566 |
<I/σ(I)> | 15.1 |
Completeness [%] | 88.6 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.1 M TRIS (pH 7.0), 12 % (w/v) PEG 6000, 1.2 M sodium chloride |