5HB2

Crystal Structure of Chaetomium thermophilum Nic96 SOL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	53.229, 73.007, 122.802
Unit cell angles	90.00, 93.60, 90.00

Refinement procedure

Resolution	47.664 - 3.300
R-factor	0.2354
Rwork	0.231
R-free	0.27830
RMSD bond length	0.003
RMSD bond angle	0.570
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.300
Number of reflections	14334
<I/σ(I)>	12
Completeness [%]	100.0
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	294	4 % Tacsimate (pH 7.4), 14 % (w/v) PEG 3350