5HB0

Crystal structure of Chaetomium thermophilum Nup170 CTD-Nup145N complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 1
Unit cell lengths	75.590, 111.470, 111.750
Unit cell angles	91.82, 92.47, 91.36

Refinement procedure

Resolution	45.364 - 3.500
R-factor	0.2124
Rwork	0.210
R-free	0.26000
RMSD bond length	0.005
RMSD bond angle	0.616
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.500
Number of reflections	45493
<I/σ(I)>	6.2
Completeness [%]	98.8
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	12 % (w/v) PEG 3350, 0.2 M lithium acetate