5HAZ

Structure of Chaetomium thermophilum Nup170 CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	141.260, 54.770, 108.200
Unit cell angles	90.00, 91.05, 90.00

Refinement procedure

Resolution	37.255 - 2.100
R-factor	0.2068
Rwork	0.206
R-free	0.23060
RMSD bond length	0.005
RMSD bond angle	0.626
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	47908
<I/σ(I)>	21
Completeness [%]	97.8
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	0.1 M HEPES (pH 7.0), 1.0 M sodium acetate