5HAY
Crystal structure of Chaetomium thermophilum Nup170 CTD Y905M L1007M L1183M V1292M mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.790, 101.700, 193.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.185 - 2.800 |
R-factor | 0.2113 |
Rwork | 0.209 |
R-free | 0.25070 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.555 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 30966 |
<I/σ(I)> | 22.4 |
Completeness [%] | 98.2 |
Redundancy | 16.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 294 | 0.1 M MES (pH 6.3), 10 % PEG 20000, 10 % (v/v) ethylene glycol, 0.2 M potassium thiocyanate |