5HAX

Crystal structure of Chaetomium thermophilum Nup170 NTD-Nup53 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	69.230, 106.160, 120.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.749 - 2.100
R-factor	0.1926
Rwork	0.191
R-free	0.22740
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	52358
<I/σ(I)>	20.3
Completeness [%]	100.0
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	294	1.0 M Na/K phosphate (pH 6.9)