5HAS
Crystal structure of the N-terminal DCB-HUS domain of T. terrestris Sec7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | .987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.472, 132.024, 247.664 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.184 - 2.653 |
| R-factor | 0.2137 |
| Rwork | 0.212 |
| R-free | 0.25680 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.232 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.155 | 0.115 | |
| Total number of observations | 569136 | ||
| Number of reflections | 59678 | ||
| <I/σ(I)> | 10.6 | ||
| Completeness [%] | 99.3 | 99.7 | 91.3 |
| Redundancy | 9.5 | 12.4 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 5% Jeffamine ED2001, 150 mM MES pH 6, 6% DMSO |
