5HAM
Structure of Rickettsia bellii effector protein RickCE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9174 |
| Spacegroup name | H 3 |
| Unit cell lengths | 173.496, 173.496, 55.516 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.075 - 2.400 |
| R-factor | 0.167 |
| Rwork | 0.164 |
| R-free | 0.21690 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.080 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.085 | 0.596 |
| Number of reflections | 24203 | |
| <I/σ(I)> | 9.5 | 2 |
| Completeness [%] | 99.2 | 99.7 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tris (pH 7.5), 2 M Ammonium citrate |
