5HAC

Crystal structure of Proliferating Cell Nuclear Antigen from Leishmania donovani at 2.95 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-05
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	133.992, 150.185, 170.079
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.950
R-factor	0.20501
Rwork	0.205
R-free	0.23234
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cfk
RMSD bond length	0.017
RMSD bond angle	2.291
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.960
High resolution limit [Å]	2.950	2.950
Number of reflections	64552
<I/σ(I)>	14.2	0.6
Completeness [%]	76.3	86.8
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3350, SODIUM MALONATE