5H9Q
Crystal Structure of Human Galectin-7 in Complex with TD139
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 53.896, 64.426, 70.616 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 27.651 - 1.931 |
| R因子 | 0.194 |
| Rwork | 0.189 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkz |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.001 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.000 |
| 分解能 [Å] (高) | 1.930 | 1.930 |
| Rmerge_l_obs | 0.036 | 0.152 |
| 独立反射数 | 19082 | |
| <I/σ(I)> | 44.9 | 11.86 |
| 完全性 [%] | 99.6 | 96.5 |
| 冗長性 | 6 | 6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.22 M Mg Acetate, 21% (w/v) PEG3350 |
