5H94
Crystal structure of Swine MHC CLASSI for 1.48 angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97892 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.453, 41.471, 106.735 |
| Unit cell angles | 90.00, 117.46, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.480 |
| R-factor | 0.15177 |
| Rwork | 0.150 |
| R-free | 0.19025 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qq4 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | PHASER |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.518 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.384 | |
| Number of reflections | 127299 | |
| <I/σ(I)> | 19.482 | 3.536 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20%(w/v) Polyethylene glycol 3350, 0.2M Ammonium fluoride |
