5H92
Crystal structure of the complex between maize Sulfite Reductase and ferredoxin in the form-3 crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-02-20 |
Detector | MACSCIENCE |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.414, 103.406, 122.434 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.270 - 2.080 |
R-factor | 0.18212 |
Rwork | 0.179 |
R-free | 0.23189 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h8v |
RMSD bond length | 0.016 |
RMSD bond angle | 1.751 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.080 |
Number of reflections | 579880 |
<I/σ(I)> | 34.9 |
Completeness [%] | 100.0 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 28% PEG4000, 0.05M Sodium Acetate |