5H8Z

Crystal structure of the C49A C353A mutant Fenna-Matthews-Olson Protein from Chlorobaculum Tepidum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-04
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 43 3 2
Unit cell lengths	168.358, 168.358, 168.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.841 - 1.800
R-factor	0.1602
Rwork	0.159
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eni
RMSD bond length	0.015
RMSD bond angle	1.505
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.113	0.753
Number of reflections	75406
<I/σ(I)>	63.9	6.4
Completeness [%]	100.0
Redundancy	48.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG 4000, 20% 2-proponal, sodium citrate, sodium chloride