5H8V
Crystal structure of the complex between maize Sulfite Reductase and ferredoxin in the form-1 crystal
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 103.346, 103.346, 255.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.040 - 2.200 |
| R-factor | 0.20075 |
| Rwork | 0.200 |
| R-free | 0.23857 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.228 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 634871 |
| <I/σ(I)> | 25.8 |
| Completeness [%] | 99.4 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 25.5 % PEG4000, 0.17M sodium acetate |
