5H8A
Mmi1 YTH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.815, 79.562, 195.193 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.798 - 1.751 |
R-factor | 0.1879 |
Rwork | 0.187 |
R-free | 0.20460 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.797 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.750 |
Number of reflections | 78868 |
<I/σ(I)> | 24.4 |
Completeness [%] | 99.7 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.1 M lithium sulfate, 0.1 M Tris (pH 8.5), 25% PEG 4000 |