5H88
Crystal structure of mRojoA mutant - T16V -P63F - W143A - L163V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.380, 94.710, 78.941 |
Unit cell angles | 90.00, 98.50, 90.00 |
Refinement procedure
Resolution | 30.160 - 2.060 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.21000 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.160 |
High resolution limit [Å] | 2.060 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 100mM HEPES and 25% Peg3350 |