5H80
Biotin Carboxylase domain of single-chain bacterial carboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-25 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 65 |
Unit cell lengths | 138.100, 138.100, 97.870 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 75.740 - 1.700 |
R-factor | 0.15084 |
Rwork | 0.149 |
R-free | 0.18441 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dzd |
RMSD bond length | 0.025 |
RMSD bond angle | 2.371 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.740 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.095 | |
Number of reflections | 116236 | |
<I/σ(I)> | 15.61 | 1.18 |
Completeness [%] | 99.9 | 97.8 |
Redundancy | 10.1 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | MgCl2 HEPES (pH 7.5) poly(acrylic acid sodium salt) 5,100. |