5H7Z
Apo structure of immunity protein TplEi of T6SS from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97876 |
| Spacegroup name | I 41 |
| Unit cell lengths | 182.227, 182.227, 78.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.252 - 3.057 |
| R-factor | 0.2053 |
| Rwork | 0.203 |
| R-free | 0.24480 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.872 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.252 | 3.240 |
| High resolution limit [Å] | 3.057 | 3.060 |
| Rmerge | 0.098 | 0.559 |
| Number of reflections | 46474 | |
| <I/σ(I)> | 14.44 | 2.67 |
| Completeness [%] | 96.9 | 81.4 |
| Redundancy | 4.28 | 3.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.52M LI2SO4, 13% peg8000 |
