5H7Y
Structure of immunity protein TplEi of T6SS from Pseudomonas aeruginosa complexed with "L" peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97876 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 113.614, 113.614, 128.131 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.798 - 2.195 |
R-factor | 0.1839 |
Rwork | 0.182 |
R-free | 0.22190 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.188 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.130 | 2.330 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.090 | 0.583 |
Number of reflections | 25539 | |
<I/σ(I)> | 26.69 | 5.51 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 16.22 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 0.1 M Sodium HEPES, 20%(w/v) PEG 10000 |