5H7W
Crystal structure of 5'-nucleotidase from venom of Naja atra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.089, 68.149, 139.815 |
| Unit cell angles | 90.00, 95.73, 90.00 |
Refinement procedure
| Resolution | 24.980 - 1.900 |
| R-factor | 0.157 |
| Rwork | 0.155 |
| R-free | 0.19700 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.136 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.076 | 0.754 |
| Number of reflections | 88970 | |
| <I/σ(I)> | 22.9 | 2.6 |
| Completeness [%] | 93.0 | 89.4 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M calcium acetate, 20% PEG 3350 |
